Journal: Cells

Article Title: Contribution of Oligodendrocytes, Microglia, and Astrocytes to Myelin Debris Uptake in an Explant Model of Inflammatory Demyelination in Rats

doi: 10.3390/cells12172203

Figure Lengend Snippet: Coexpression of Olig2 with LRP1 and MBP in the CA1 region during LPS + IFN-γ-induced demyelination. ( A ) Confocal images displaying coexpression of Olig2 (green) and LRP1 (red) in explants under control conditions and after LPS + IFN-γ treatment for 1 day. Nuclei are stained with DAPI (blue). Arrows point to intensely stained Olig2 + /LRP1 + cells at 1 day. Scale bars: 20 μm. B, left panels, Representative confocal images showing Olig2 (green) and MBP (red) coexpression under control conditions and after LPS + IFN-γ exposure for 1, 2, and 3 days. ( B ) right panels, Higher magnification images displaying representative double-labeled Olig2 + /MBP + cells observed at day 1, day 2, and day 3. Note the accumulation of MBP immunoreactivity at peri-membrane soma sites of Olig2 + cells at day 2 and within the cytosol at day 3. Scale bars: 20 μm. ( C ) Quantitative analysis of double-labeled LRP1 + /Olig2 + cells in slices under control conditions and after LPS + IFN-γ treatment for 1 day. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, LPS + IFN-γ versus control. ( D ) Quantitative analysis of the number of double-labeled Olig2 + /MBP + scored according to somatic MBP immunoreactivity at day 1, day 2, and day 3. The values represent the mean ± S.E.M. (n = 3). * p < 0.05, peri-MBP + versus peri-MBP ++ and cyto-MBP at 1 day; * p < 0.05, peri-MBP ++ versus peri-MBP + and cyto-MBP at 2 days; * p < 0.05, cyto-MBP versus peri-MBP + and peri-MBP ++ at 3 days.

Article Snippet: After fixation and blocking, slices were incubated with the indicated primary antibodies for 48 h at 4 °C: rat monoclonal anti-MBP (1:1000, Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-Olig2 (1:200, Merck-Life Science Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-NG2 (1:400, Merck-Life Science S.r.l., Milan, Italy), rabbit polyclonal anti-Iba1 (1:2000, FUJIFILM Wako Chemicals, Osaka, Japan), rabbit polyclonal anti-GFAP (1:1000, Merck-Life Science, Merck-Life Science S.r.l., Milan, Italy), and mouse monoclonal anti-low density lipoprotein receptor-related protein 1 (LRP1, 1:200 Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Staining, Labeling, Membrane

Journal: Cureus

Article Title: Role of Facial Nerve Reconstruction With Anastomosis and Polyglycolic Acid Tube in Accelerating Functional Recovery After Axotomy in the Rat Facial Nucleus

doi: 10.7759/cureus.57326

Figure Lengend Snippet: (A, B) Cross-sections of facial nerves immunostained with MBP (green), β3-tubulin (red), and DAPI (blue). Scale bar: 10 μm (A), magnified view. Scale bar: 100 μm (B). (C) Number of myelinated fibers per area relative to the post-operative date. Data are expressed as mean ± standard error of the mean. N = 4. *p < 0.05; **p < 0.01 as determined based on analysis of variance and post-hoc Tukey’s test. DAPI, 4′,6-diamidino-2-phenylindole; MBP, myelin basic protein

Article Snippet: Histology To evaluate axonal degeneration and demyelination, we processed the 14-μm sections for immunostaining using antibodies against myelin basic protein (MBP) (1:400; Merck Millipore, Burlington, MA, USA) and β3-tubulin (1:400; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: